CCVR Proteomics Core
Director Tomoyoshi Kobayashi, Ph.D.

The proteomic core conducts and/or consults on the following major experimental approaches for analysis and characterization of proteins within biological samples:

  • SDS-PAGE
  • 1D-NEIEF
  • 2D-Electrophoresis
  • MALDI-TOF
  • MS/MS.

The proteomic core has access to the following equipments and softwares (with or without fee):

  • BioRad Protean IEF cell along with the 2nd dimension gel boxes for 2D gels.
  • Imagequant software for 1D gel analysis.
  • Quantity One software for 1D gel analysis.
  • PDQest software for 2D-gel analysis.
  • Typhoon 9410 imager for DIGE imaging.
  • Biorad FX Pro Plus imager for imaging both 1Dand 2D gels.
  • Dionex Analytical HPLC U-3000.
  • Dionex Capilary HPLC U-3000.
  • Voyager DE Pro Maldi-TOF (Applied Biosystems).
  • Thermo Scientific 2D-LC with the LTQ mass spectrometer.
  • Thermo Scientific LTQ-FT mass spectrometer.
  • Mascot, Seaquest, Bioworks, and Scaffold software for mass spectrometry data analysis software.